Title
Biological control of Rhizoctonia root rot on bean by phenazine and cyclic lipopeptide producing Pseudomonas CMR12a Biological control of Rhizoctonia root rot on bean by phenazine and cyclic lipopeptide producing Pseudomonas CMR12a
Author
Faculty/Department
Faculty of Applied Engineering Sciences
Publication type
article
Publication
Lancaster, Pa ,
Subject
Biology
Engineering sciences. Technology
Source (journal)
Phytopathology. - Lancaster, Pa
Volume/pages
101(2011) :8 , p. 996-1004
ISSN
0031-949X
ISI
000292989900012
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Abstract
Pseudomonas CMR12a was previously selected as an efficient biocontrol strain producing phenazines and cyclic lipopeptides (CLPs). In this study, biocontrol capacity of Pseudomonas CMR12a against Rhizoctonia root rot of bean and the involvement of phenazines and CLPs in this ability were tested. Two different anastomosis groups (AGs) of Rhizoctonia solani, the intermediately aggressive AG 2-2 and the highly aggressive AG 4 HGI, were included in growth-chamber experiments with bean plants. The wild-type strain CMR12a dramatically reduced disease severity caused by both R. solani AGs. A CLP-deficient and a phenazine-deficient mutant of CMR12a still protected bean plants, albeit to a lesser extent compared with the wild type. Two mutants deficient in both phenazine and CLP production completely lost their biocontrol activity. Disease-suppressive capacity of CMR12a decreased after washing bacteria before application to soil and thereby removing metabolites produced during growth on plate. In addition, microscopic observations revealed pronounced branching of hyphal tips of both R. solani AGs in the presence of CMR12a. More branched and denser mycelium was also observed for the phenazine-deficient mutant; however, neither the CLP-deficient mutant nor the mutants deficient in both CLPs and phenazines influenced hyphal growth. Together, results demonstrate the involvement of phenazines and CLPs during Pseudomonas CMR12a-mediated biocontrol of Rhizoctonia root rot of bean.
Full text (open access)
https://repository.uantwerpen.be/docman/irua/592f1a/1a2f249b.pdf
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