Title
Organization of the BcgI restriction-modification protein for the transfer of one methyl group to DNAOrganization of the BcgI restriction-modification protein for the transfer of one methyl group to DNA
Author
Faculty/Department
Faculty of Sciences. Chemistry
Research group
Biomolecular & Analytical Mass Spectrometry (BAMS)
Publication type
article
Publication
London,
Subject
Chemistry
Source (journal)
Nucleic acids research. - London
Volume/pages
41(2013):1, p. 405-417
ISSN
0305-1048
ISI
000312889900065
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Affiliation
University of Antwerp
Abstract
The Type IIB restrictionmodification protein BcgI contains A and B subunits in a 2:1 ratio: A has the active sites for both endonuclease and methyltransferase functions while B recognizes the DNA. Like almost all Type IIB systems, BcgI needs two unmethylated sites for nuclease activity; it cuts both sites upstream and downstream of the recognition sequence, hydrolyzing eight phosphodiester bonds in a single synaptic complex. This complex may incorporate four A2B protomers to give the eight catalytic centres (one per A subunit) needed to cut all eight bonds. The BcgI recognition sequence contains one adenine in each strand that can be N6-methylated. Although most DNA methyltransferases operate at both unmethylated and hemi-methylated sites, BcgI methyltransferase is only effective at hemi-methylated sites, where the nuclease component is inactive. Unlike the nuclease, the methyltransferase acts at solitary sites, functioning catalytically rather than stoichiometrically. Though it transfers one methyl group at a time, presumably through a single A subunit, BcgI methyltransferase can be activated by adding extra A subunits, either individually or as part of A2B protomers, which indicates that it requires an assembly containing at least two A2B units.
Full text (open access)
https://repository.uantwerpen.be/docman/irua/f694f3/e2747039.pdf
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