Title
Quantitative electron tomography : the effect of the three-dimensional point spread function Quantitative electron tomography : the effect of the three-dimensional point spread function
Author
Faculty/Department
Faculty of Sciences. Physics
Publication type
article
Publication
Amsterdam ,
Subject
Physics
Chemistry
Source (journal)
Ultramicroscopy. - Amsterdam
Volume/pages
135(2013) , p. 1-5
ISSN
0304-3991
ISI
000326941500001
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Affiliation
University of Antwerp
Abstract
The intensity levels in a three-dimensional (3D) reconstruction, obtained by electron tomography, can be influenced by several experimental imperfections. Such artifacts will hamper a quantitative interpretation of the results. In this paper, we will correct for artificial intensity variations by determining the 3D point spread function (PSF) of a tomographic reconstruction based on high angle annular dark field scanning transmission electron microscopy. The large tails of the PSF cause an underestimation of the intensity of smaller particles, which in turn hampers an accurate radius estimate. Here, the error introduced by the PSF is quantified and corrected a posteriori.
E-info
https://repository.uantwerpen.be/docman/iruaauth/24fddc/12722e4bb79.pdf
Full text (open access)
https://repository.uantwerpen.be/docman/irua/56fad6/8371.pdf
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