Title
Potentiometric sensors doped with biomolecules as a new approach to small molecule/biomolecule binding kinetics analysisPotentiometric sensors doped with biomolecules as a new approach to small molecule/biomolecule binding kinetics analysis
Author
Faculty/Department
Faculty of Sciences. Biology
Faculty of Sciences. Chemistry
Faculty of Pharmaceutical, Biomedical and Veterinary Sciences . Biomedical Sciences
Research group
Integrated Molecular Plant Physiology Research (IMPRES)
AXES (Antwerp X-ray Analysis, Electrochemistry and Speciation)
Department of Chemistry - other
Human molecular genetics
Publication type
article
Publication
Barking,
Subject
Physics
Chemistry
Biology
Engineering sciences. Technology
Source (journal)
Biosensors and bioelectronics. - Barking, 1990, currens
Volume/pages
54(2014), p. 515-520
ISSN
0956-5663
ISI
000333071500077
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Affiliation
University of Antwerp
Abstract
The most successful binding kinetics analysis systems at this moment include surface plasmon resonance (SPR), quartz microcrystal balance (QMB) and surface acoustic wave (SAW). Although these are powerful methods, they generally are complex, expensive and require the use of monolayers. Here, we report on potentiometric sensors as an inexpensive and simple alternative to do binding kinetics analysis between small molecules in solution and biomolecules (covalently) attached in a biopolymer sensor coating layer. As an example, dopamine and an anti-dopamine aptamer were used as the small molecule and the biomolecule respectively. Binding between both follows a Langmuir adsorption type model and creates a surface potential. The system operates in Flow Injection Analysis mode (FIA). Besides being an interesting new binding kinetics tool, the approach allows systematic design of potentiometric biosensors (in the present study a dopamine sensor), and gives new insights into the functioning of ion-selective electrodes (ISEs).
E-info
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