Title
Osmium tetroxide labeling of (poly)methyl methacrylate corrosion casts for enhancement of micro-CT microvascular imaging Osmium tetroxide labeling of (poly)methyl methacrylate corrosion casts for enhancement of micro-CT microvascular imaging
Author
Faculty/Department
Faculty of Pharmaceutical, Biomedical and Veterinary Sciences. Veterinary Sciences
Publication type
article
Publication
Cambridge, Mass. ,
Subject
Physics
Chemistry
Veterinary medicine
Source (journal)
Microscopy and microanalysis. - Cambridge, Mass.
Volume/pages
19(2013) :6 , p. 1416-1427
ISSN
1431-9276
ISI
000330344800005
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Affiliation
University of Antwerp
Abstract
In order to enhance micro-computer tomography (micro-CT) imaging of corrosion casts of fine vasculature, metals can be added to the casting resin before perfusion. However, perfused metals lead to vasoconstriction or vessel damage resulting in nonphysiologic vascular casts. A novel method for coating methyl methacrylate vascular casts with osmium tetroxide has been developed in order to increase micro-CT contrast without affecting the vascular structure. This technique was verified using corrosion casts of the lung vasculature of New Zealand white rabbits. Osmium tetroxide coating of methyl methacrylate vascular corrosion casts resulted in an increase in overall sample contrast that translated into an increase in the resolution of the vasculature. This method can therefore lead to increased resolution in the characterization of fine vascular structures.
E-info
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