Title
The thiol compounds glutathione and homoglutathione differentially affect cell development in alfalfa (**Medicago sativa** L.) The thiol compounds glutathione and homoglutathione differentially affect cell development in alfalfa (**Medicago sativa** L.)
Author
Faculty/Department
Faculty of Sciences. Biology
Faculty of Sciences. Bioscience Engineering
Publication type
article
Publication
Paris ,
Subject
Biology
Engineering sciences. Technology
Source (journal)
Plant physiology and biochemistry. - Paris, 1987, currens
Volume/pages
74(2014) , p. 16-23
ISSN
0981-9428
ISI
000331848900003
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Affiliation
University of Antwerp
Abstract
Glutathione (GSH) is an important scavenger of Reactive Oxygen Species (ROS), precursor of metal chelating phytochelatins, xenobiotic defence compound and regulator of cell proliferation. Homoglutathione (hGSH) is a GSH homologue that is present in several taxa in the family of Fabaceae. It is thought that hGSH performs many of the stress-defence roles typically ascribed to GSH, yet little is known about the potential involvement of hGSH in controlling cell proliferation. Here we show that hGSH/GSH ratios vary across organs and cells and that these changes in hGSH/GSH ratio occur during dedifferentiation and/or cell cycle activation events. The use of a GSH/hGSH biosynthesis inhibitor resulted in impaired cytokinesis in isolated protoplasts, showing the critical importance of these thiol-compounds for cell division. However, exposure of isolated protoplasts to exogenous GSH accelerated cytokinesis, while exogenous hGSH was found to inhibit the same process. We conclude that GSH and hGSH have distinct functional roles in cell cycle regulation in Medicago sativa L. GSH is associated with meristemic cells, and promotes cell cycle activation and induction of somatic embryogenesis, while hGSH is associated with differentiated cells and embryo proliferation.
E-info
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