Title
Measurement of S-phase duration of adult stem cells in the flatworm **Macrostomum lignano** by double replication labelling and quantitative colocalization analysis Measurement of S-phase duration of adult stem cells in the flatworm **Macrostomum lignano** by double replication labelling and quantitative colocalization analysis
Author
Faculty/Department
Faculty of Pharmaceutical, Biomedical and Veterinary Sciences . Biomedical Sciences
Publication type
article
Publication
London ,
Subject
Biology
Human medicine
Source (journal)
Cell biology international. - London, 1993, currens
Volume/pages
36(2012) :12 , p. 1251-1259
ISSN
1065-6995
1095-8355
ISI
000311144500019
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Abstract
Platyhelminthes are highly attractive models for addressing fundamental aspects of stem cell biology in vivo. These organisms possess a unique stem cell system comprised of neoblasts that are the only proliferating cells during adulthood. We have investigated T-s (S-phase duration) of neoblasts during homoeostasis and regeneration in the flatworm, Macrostomum lignano. A double immunohistochemical technique was used, performing sequential pulses with the thymidine analogues CldU (chlorodeoxyuridine) and IdU (iododeoxyuridine), separated by variable chase times in the presence of colchicine. Owing to the localized nature of the fluorescent signals (cell nuclei) and variable levels of autofluorescence, standard intensity-based colocalization analyses could not be applied to accurately determine the colocalization. Therefore, an object-based colocalization approach was devised to score the relative number of double-positive cells. Using this approach, T-s (S-phase duration) in the main population of neoblasts was similar to 13 h. During early regeneration, no significant change in T-s was observed.
E-info
https://repository.uantwerpen.be/docman/iruaauth/45047c/7ac5952.pdf
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