Title
Simulated microgravity decreases apoptosis in fetal fibroblasts Simulated microgravity decreases apoptosis in fetal fibroblasts
Author
Faculty/Department
Faculty of Pharmaceutical, Biomedical and Veterinary Sciences . Biomedical Sciences
Publication type
article
Publication
Athens ,
Subject
Human medicine
Source (journal)
International journal of molecular medicine. - Athens
Volume/pages
30(2012) :2 , p. 309-313
ISSN
1107-3756
ISI
000306155400014
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Abstract
Space travel is a major challenge for human beings. Especially, the mechanisms through which space conditions might alter animal development have been questioned for a long time. The two major physical stress factors that are of relevance in this context are space radiation and weightlessness. While it has been extensively shown that high doses of ionizing radiation induce deleterious effects on embryonic development, so far, little is known about the potential harmful effects of radiation in combination with microgravity on the developing organism. In the present study, we investigated the effects of simulated microgravity on irradiated STO mouse fetal fibroblast cells using a random positioning machine (RPM). Radiation-induced cell cycle changes were not affected when cells were subjected to simulated microgravity for 24 h. Moreover, no morphological differences were observed in irradiated samples exposed to simulated microgravity compared to cells that were exclusively irradiated. However, microgravity simulation significantly decreased the level of apoptosis at all doses as measured by caspase-3 activity and it prevented cells from undergoing radiation-induced size increase up to 1 Gy.
E-info
https://repository.uantwerpen.be/docman/iruaauth/613f65/a405954.pdf
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