Publication
Title
Repeated exposure of human fibroblasts to ionizing radiation reveals an adaptive response that is not mediated by interleukin-6 or TGF-
Author
Abstract
Exposing cells to a low dose can protect them against a subsequent higher exposure. This phenomenon is known as adaptive response and is frequently observed in a variety of cells. Even though similarities are suspected with other non-targeted effects, such as bystander effects, the exact mechanism behind adaptive response is not fully clarified. In this study human primary fibroblasts were tested for their response to ionizing radiation (IR) after administrating a low priming dose (0.1-0.5 Gy). Both the abundance of gamma H2AX as a marker for double-stranded breaks and the levels of cytokines, secreted in the medium, were monitored in time. Upon challenge, IR-primed cells showed modified gamma H2AX spot size distributions and altered repair kinetics, consistent with an adaptive response. In addition, 24 h after priming with IR, four cytokines were significantly upregulated in the medium - GM-CSF (1.33 x); IL6 (4.24 x); IL8 (1.33x); TGF-beta (1.46x). In order to mimick the protective effect of IR priming, we primed the cells with either IL6 or TGF-beta. This did not elicit an altered gamma H2AX response as observed in IR-primed cells, indicating that the adaptive response in these primary fibroblasts is regulated in an IL-6 and TGF-beta independent manner. (C) 2011 Elsevier B.V. All rights reserved.
Language
English
Source (journal)
Mutation research: international journal on mutagenesis, chromosome breakage and related subjects. - Amsterdam
Publication
Amsterdam : 2011
ISSN
0027-5107
Volume/pages
715:1-2(2011), p. 19-24
ISI
000295664200004
Full text (Publisher's DOI)
Full text (publisher's version - intranet only)
UAntwerpen
Faculty/Department
Publication type
Subject
External links
Web of Science
Record
Identification
Creation 11.12.2013
Last edited 11.10.2017