Title
2D mapping of strongly deformable cell nuclei-based on contour matching2D mapping of strongly deformable cell nuclei-based on contour matching
Author
Faculty/Department
Faculty of Pharmaceutical, Biomedical and Veterinary Sciences . Biomedical Sciences
Publication type
article
Publication
New York,
Subject
Chemistry
Biology
Human medicine
Source (journal)
Cytometry: part A. - New York, 2003, currens
Volume/pages
79A(2011):7, p. 580-588
ISSN
1552-4922
ISI
000292947900010
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Abstract
The spatiotemporal dynamics of protein complexes and genome loci are functionally linked to cellular health status. To study the inherent motion of subnuclear particles, it is essential to remove any superimposed component stemming from displacement and deformation of the nucleus. In this article, we propose a mapping of the nuclear interior, which is based on the deformation of the nuclear contour and has no shape constraints. This registration procedure enabled an accurate estimation of telomere mobility in living human cells undergoing dramatic nuclear deformations. Given the large variety of pathologies and cellular processes that are associated with strong nuclear shape changes, the contour mapping algorithm has generic value for improving the accuracy of mobility measurements of genome loci and intranuclear macromolecule complexes. (C) 2011 International Society for Advancement of Cytometry
E-info
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