Publication
Title
2D mapping of strongly deformable cell nuclei-based on contour matching
Author
Abstract
The spatiotemporal dynamics of protein complexes and genome loci are functionally linked to cellular health status. To study the inherent motion of subnuclear particles, it is essential to remove any superimposed component stemming from displacement and deformation of the nucleus. In this article, we propose a mapping of the nuclear interior, which is based on the deformation of the nuclear contour and has no shape constraints. This registration procedure enabled an accurate estimation of telomere mobility in living human cells undergoing dramatic nuclear deformations. Given the large variety of pathologies and cellular processes that are associated with strong nuclear shape changes, the contour mapping algorithm has generic value for improving the accuracy of mobility measurements of genome loci and intranuclear macromolecule complexes. (C) 2011 International Society for Advancement of Cytometry
Language
English
Source (journal)
Cytometry: part A. - New York, 2003, currens
Publication
New York : 2011
ISSN
1552-4922
Volume/pages
79A:7(2011), p. 580-588
ISI
000292947900010
Full text (Publisher's DOI)
Full text (publisher's version - intranet only)
UAntwerpen
Faculty/Department
Publication type
Subject
External links
Web of Science
Record
Identification
Creation 11.12.2013
Last edited 07.08.2017