Title
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Internalization of **Sambucus nigra** agglutinins I and II in insect midgut CF-203 cells
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Author
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Abstract
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In this project, the uptake mechanisms and localization of two lectins from Sambucus nigra, further referred to as S. nigra agglutinin (SNA)-I and SNA-II, into insect midgut CF-203 cells were studied. SNA-I is a chimeric lectin belonging to the class of ribosome-inactivating proteins, whereas SNA-II is a hololectin devoid of enzymatic activity. Internalization of the fluorescein isothiocyanate-labeled lectin was investigated using confocal microscopy. Both lectins were internalized into the cytoplasm of CF-203 cells at similar rates. Preexposure of the insect midgut cells to specific inhibitors of clathrin-and caveolae-mediated endocytosis resulted in an inhibition of lectin uptake in CF-203 cells and caspase-induced cytotoxicity caused by SNA-I and SNA-II, confirming the involvement of both endocytosis pathways. Further studies demonstrated that the uptake mechanism(s) for both lectins required phosphoinositide 3-kinases, but did not depend on the actin cytoskeleton. Since the hololectin SNA-II apparently uses a similar endocytosis pathway as the chimerolectin SNA-I, it can be concluded that the endocytosis process mainly relies on the carbohydrate-binding activity of the lectins under investigation. (C) 2011 Wiley Periodicals, Inc. |
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Language
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English
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Source (journal)
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Archives of insect biochemistry and physiology. - New York
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Publication
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New York
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2011
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ISSN
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0739-4462
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DOI
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10.1002/ARCH.20405
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Volume/pages
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76
:4
(2011)
, p. 211-222
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ISI
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000288543400003
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Full text (Publisher's DOI)
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Full text (publisher's version - intranet only)
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