Title
Increased plasticity of the nuclear envelope and hypermobility of telomeres due to the loss of A-type lamins
Author
Faculty/Department
Faculty of Pharmaceutical, Biomedical and Veterinary Sciences . Biomedical Sciences
Publication type
article
Publication
Amsterdam ,
Subject
Physics
Chemistry
Biology
Source (journal)
Biochimica et biophysica acta : G : general subjects. - Amsterdam
Volume/pages
1800(2010) :4 , p. 448-458
ISSN
0304-4165
ISI
000275843100004
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Abstract
Background: The nuclear lamina provides structural support to the nucleus and has a central role in defining nuclear organization. Defects in its filamentous constituents, the lamins, lead to a class of diseases collectively referred to as laminopathies. On the cellular level, lamin mutations affect the physical integrity of nuclei and nucleo-cytoskeletal interactions, resulting in increased susceptibility to mechanical stress and altered gene expression. Methods: In this study we quantitatively compared nuclear deformation and chromatin mobility in fibroblasts from a homozygous nonsense LMNA mutation patient and a Hutchinson-Gilford progeria syndrome patient with wild type dermal fibroblasts, based on the visualization of mCitrine labeled telomere-binding protein TRF2 with light-economical imaging techniques and cytometric analyses. Results: Without application of external forces, we found that the absence of functional lamin A/C leads to increased nuclear plasticity on the hour and minute time scale but also to increased intranuclear mobility down to the second time scale. In contrast, progeria cells show overall reduced nuclear dynamics. Experimental manipulation (farnesyltransferase inhibition or lamin A/C silencing) confirmed that these changes in mobility are caused by abnormal or reduced lamin A/C expression. Conclusions: These observations demonstrate that A-type lamins affect both nuclear membrane and telomere dynamics. General significance: Because of the pivotal role of dynamics in nuclear function, these differences likely Contribute to or represent novel mechanisms in laminopathy development. (c) 2010 Elsevier B.V. All rights reserved.
E-info
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