Title
In vitro susceptibility of Leishmania donovani to Miltefosine in Indian Visceral Leishmaniasis In vitro susceptibility of Leishmania donovani to Miltefosine in Indian Visceral Leishmaniasis
Author
Faculty/Department
Faculty of Pharmaceutical, Biomedical and Veterinary Sciences . Biomedical Sciences
Publication type
article
Publication
Baltimore, Md ,
Subject
Human medicine
Source (journal)
The American journal of tropical medicine and hygiene. - Baltimore, Md
Volume/pages
89(2013) :4 , p. 750-754
ISSN
0002-9637
ISI
000326122100021
Carrier
E
Target language
English (eng)
Affiliation
University of Antwerp
Abstract
Promastigote miltefosine (MIL) susceptibility was performed on Leishmania donovani isolates from Indian patients with visceral leishmaniasis treated with MIL. Isolates that were obtained before the onset of MIL treatment, after completion of treatment (29th day), or at the time of treatment failure, were screened using in vitro promastigote assay. The MIL susceptibility of the pre-treatment isolates (N = 24, mean IC50 +/- SEM = 3.74 +/- 0.38 mu M) was significantly higher than that of the post-treatment group (N = 26, mean IC50 +/- SEM = 6.15 +/- 0.52 mu M; P = 0.0006) but was similar in the cured patients (N = 22, mean IC50 +/- SEM = 5.58 +/- 0.56 mu M) and those who failed treatment (N = 28, mean IC50 +/- SEM = 4.53 +/- 0.47 mu M). The pre/post-treatment results thus showed a 2-fold difference, whereas isolated from cured versus failed patients showed a similar susceptibility, suggesting that this higher tolerance is not responsible for MIL-treatment failure. Our work highlights the need for careful monitoring of MIL susceptibility for implementation in national VL elimination programs.
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