Title
Surface enhanced Raman optical activity as an ultra sensitive tool for ligand binding analysis Surface enhanced Raman optical activity as an ultra sensitive tool for ligand binding analysis
Author
Faculty/Department
Faculty of Sciences. Chemistry
Publication type
article
Publication
Ottawa, Ont. ,
Subject
Chemistry
Biology
Source (journal)
Spectroscopy. - Ottawa, Ont.
Volume/pages
21(2007) :3 , p. 143-149
ISSN
0712-4813
ISI
000249884900001
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Abstract
The Surface Enhanced Resonance Raman Scattering (SERRS) and Surface Enhanced Resonance Raman Optical Activity (SERROA) spectra of myoglobin and the myoglobin-azide complex were measured on very dilute samples (100 nM protein) in order to analyze the sensitivity of SERROA spectroscopy when inducing small structural changes. While the SERRS spectra of the two compounds were virtually identical, comparison of the SERROA spectra revealed several differences, including frequency shifts and changes in signal intensity, consistent with structural change in the porphyrin prosthetic group of the protein upon azide complexation. Application of this method allows for rapid analysis of ligand binding in metalloproteins in dilute aqueous solution and could in the future, when combined with theoretical studies, increase the obtainable structural resolution of proteins beyond that of X-ray analysis.
E-info
https://repository.uantwerpen.be/docman/iruaauth/64d93c/fcc6059.pdf
http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000249884900001&DestLinkType=RelatedRecords&DestApp=ALL_WOS&UsrCustomerID=ef845e08c439e550330acc77c7d2d848
http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000249884900001&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=ef845e08c439e550330acc77c7d2d848
http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000249884900001&DestLinkType=CitingArticles&DestApp=ALL_WOS&UsrCustomerID=ef845e08c439e550330acc77c7d2d848