Title
The effect of an elevated cytokinin level using the **ipt** gene and <tex>$N^{6}$</tex>-benzyladenine on single node and intact potato plant tuberization **in vitro** The effect of an elevated cytokinin level using the **ipt** gene and <tex>$N^{6}$</tex>-benzyladenine on single node and intact potato plant tuberization **in vitro**
Author
Faculty/Department
Faculty of Sciences. Biology
Publication type
article
Publication
New York ,
Subject
Biology
Source (journal)
Journal of plant growth regulation. - New York
Volume/pages
14(1995) :3 , p. 143-150
ISSN
0721-7595
ISI
A1995TG23800005
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Affiliation
University of Antwerp
Abstract
Two models of potato (Solanum tuberosum L.) tuberization in vitro (intact plants and single nodes) were used to study the role of cytokinins in this process. We applied hormone in two different ways. The exogenous addition of 10 mg . L(-1) N-6-benzyladenine (BA) into the tuberization medium resulted in advanced tuber formation in intact plants, and microtubers appeared 10-20 days earlier than in the experiments in which no cytokinin was supplied. Transformation with the Agrobacterium tumefaciens ipt gene provided potato clones with endogenously elevated cytokinin levels (3-20 times higher zeatin riboside content in different clones). The onset of tuberization in intact ipt-transformed plants with low transgene expression was advanced in comparison with control material, and exogenously applied BA further promoted the tuberization process. On the contrary, tuberization was strongly inhibited in ipt-transformed nodes, and an external increase of the cytokinin level caused complete inhibition of explant growth. In untransformed (control) nodes cytokinin application resulted in primary and secondary tuber formation, which depended on the BA concentration in cultivation media.
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