Title
|
|
|
|
Comparison of SPF10 real-time PCR and conventional PCR in combination with the INNO-LiPA HPV Genotyping Extra assay for the detection and typing of human papillomavirus in cervical samples
|
|
Author
|
|
|
|
|
|
Abstract
|
|
|
|
The novel SPF10 real-time PCR assay allows the simultaneous amplification and detection of the HPV target. That way, LiPA analysis of the HPV-negative samples can be avoided, reducing workload and cost. This study aims to evaluate the performance of the SPF10 real-time PCR in combination with the LiPA assay for HPV detection and typing in cervical samples. Thirty-nine cervical samples were subjected to the SPF10 conventional PCR in combination with the LiPA assay. Subsequently, the SPF10 real-time PCR was performed to enable the comparison between the SPF10 conventional and the real-time PCR results. In case of discrepancy, the samples were subjected to the CLAR(R) HPV2 assay. As a result, 27 out of 39 samples were identified as HPV-positive by the SPF10 real-time PCR and were genotyped further by the LiPA assay. Twenty samples (74.1%) showed an absolute agreement between the conventional and real-time SPF10 PCR (concordant), three (11.1%) displayed additional or fewer types (compatible), two (7.4%) did not show any similarity between both assays (discordant) and the remaining two (7.4%) were LiPA-negative. The two assays showed an excellent strength of agreement for individual (k = 0.932) and multiple genotype detection (k = 0.834). In conclusion, the two SPF10 PCR methods are comparable. Therefore, the SPF10 real-time PCR with subsequent LiPA could be used for the detection and genotyping of HPV in cervical samples. (C) 2013 Elsevier B.V. All rights reserved. |
|
|
Language
|
|
|
|
English
|
|
Source (journal)
|
|
|
|
Journal of virological methods. - Amsterdam
|
|
Publication
|
|
|
|
Amsterdam
:
2013
|
|
ISSN
|
|
|
|
0166-0934
|
|
DOI
|
|
|
|
10.1016/J.JVIROMET.2013.08.013
|
|
Volume/pages
|
|
|
|
194
:1-2
(2013)
, p. 113-117
|
|
ISI
|
|
|
|
000328521600018
|
|
Full text (Publisher's DOI)
|
|
|
|
|
|
Full text (publisher's version - intranet only)
|
|
|
|
|
|