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Title
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DNA barcoding and the differentiation between North American and West European **Phormia regina** (Diptera, Calliphoridae, Chrysomyinae)
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Author
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Abstract
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| Phormia regina (the black fly) is a common Holarctic blow fly species which serves as a primary indicator taxon to estimate minimal post mortem intervals. It is also a major research model in physiological and neurological studies on insect feeding. Previous studies have shown a sequence divergence of up to 4.3% in the mitochondrial COI gene between W European and N American P. regina populations. Here, we DNA barcoded P. regina specimens from six N American and 17 W European populations and confirmed a mean sequence divergence of ca. 4% between the populations of the two continents, while sequence divergence within each continent was a ten-fold lower. Comparable mean mtDNA sequence divergences were observed for COII (3.7%) and cyt b (5.3%), but mean divergence was lower for 16S (0.4-0.6%). Intercontinental divergence at nuclear DNA was very low (<= 0.1% for both 28S and ITS2), and we did not detect any morphological differentiation between N American and W European specimens. Therefore, we consider the strong differentiation at COI, COII and cyt b as intraspecific mtDNA sequence divergence that should be taken into account when using P. regina in forensic casework or experimental research. |
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Language
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English
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Source (journal)
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ZooKeys
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Publication
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2013
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ISSN
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1313-2989
1313-2970
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DOI
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10.3897/ZOOKEYS.365.6202
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Volume/pages
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365
(2013)
, p. 149-174
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ISI
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000329206600010
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Full text (Publisher's DOI)
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Full text (open access)
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