Title
Quantifying the mechanical micro-environment during three-dimensional cell expansion on microbeads by means of individual cell-based modelling Quantifying the mechanical micro-environment during three-dimensional cell expansion on microbeads by means of individual cell-based modelling
Author
Faculty/Department
Faculty of Sciences. Mathematics and Computer Science
Publication type
article
Publication
,
Subject
Computer. Automation
Source (journal)
Computer methods in biomechanics and biomedical engineering. - Place of publication unknown
Volume/pages
16(2013) :10 , p. 1071-1084
ISSN
1025-5842
ISI
000325844600005
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Abstract
Controlled in vitro three-dimensional cell expansion requires culture conditions that optimise the biophysical micro-environment of the cells during proliferation. In this study, we propose an individual cell-based modelling platform for simulating the mechanics of cell expansion on microcarriers. The lattice-free, particle-based method considers cells as individual interacting particles that deform and move over time. The model quantifies how the mechanical micro-environment of individual cells changes during the time of confluency. A sensitivity analysis is performed, which shows that changes in the cell-specific properties of cell-cell adhesion and cell stiffness cause the strongest change in the mechanical micro-environment of the cells. Furthermore, the influence of the mechanical properties of cells and microbead is characterised. The mechanical micro-environment is strongly influenced by the adhesive properties and the size of the microbead. Simulations show that even in the absence of strong biological heterogeneity, a large heterogeneity in mechanical stresses can be expected purely due to geometric properties of the culture system.Supplemental data for this article can be accessed online.
E-info
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