Publication
Title
Quantifying the mechanical micro-environment during three-dimensional cell expansion on microbeads by means of individual cell-based modelling
Author
Abstract
Controlled in vitro three-dimensional cell expansion requires culture conditions that optimise the biophysical micro-environment of the cells during proliferation. In this study, we propose an individual cell-based modelling platform for simulating the mechanics of cell expansion on microcarriers. The lattice-free, particle-based method considers cells as individual interacting particles that deform and move over time. The model quantifies how the mechanical micro-environment of individual cells changes during the time of confluency. A sensitivity analysis is performed, which shows that changes in the cell-specific properties of cell-cell adhesion and cell stiffness cause the strongest change in the mechanical micro-environment of the cells. Furthermore, the influence of the mechanical properties of cells and microbead is characterised. The mechanical micro-environment is strongly influenced by the adhesive properties and the size of the microbead. Simulations show that even in the absence of strong biological heterogeneity, a large heterogeneity in mechanical stresses can be expected purely due to geometric properties of the culture system.Supplemental data for this article can be accessed online.
Language
English
Source (journal)
Computer methods in biomechanics and biomedical engineering. - Place of publication unknown
Publication
Place of publication unknown : 2013
ISSN
1025-5842
Volume/pages
16:10(2013), p. 1071-1084
ISI
000325844600005
Full text (Publisher's DOI)
UAntwerpen
Faculty/Department
Research group
Publication type
Subject
External links
Web of Science
Record
Identification
Creation 24.02.2014
Last edited 07.08.2017