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Title
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Quantifying the mechanical micro-environment during three-dimensional cell expansion on microbeads by means of individual cell-based modelling
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Author
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Abstract
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| Controlled in vitro three-dimensional cell expansion requires culture conditions that optimise the biophysical micro-environment of the cells during proliferation. In this study, we propose an individual cell-based modelling platform for simulating the mechanics of cell expansion on microcarriers. The lattice-free, particle-based method considers cells as individual interacting particles that deform and move over time. The model quantifies how the mechanical micro-environment of individual cells changes during the time of confluency. A sensitivity analysis is performed, which shows that changes in the cell-specific properties of cell-cell adhesion and cell stiffness cause the strongest change in the mechanical micro-environment of the cells. Furthermore, the influence of the mechanical properties of cells and microbead is characterised. The mechanical micro-environment is strongly influenced by the adhesive properties and the size of the microbead. Simulations show that even in the absence of strong biological heterogeneity, a large heterogeneity in mechanical stresses can be expected purely due to geometric properties of the culture system.Supplemental data for this article can be accessed online. |
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Language
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English
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Source (journal)
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Computer methods in biomechanics and biomedical engineering. - Place of publication unknown
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Publication
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Place of publication unknown
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2013
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ISSN
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1025-5842
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DOI
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10.1080/10255842.2013.829461
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Volume/pages
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16
:10
(2013)
, p. 1071-1084
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ISI
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000325844600005
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Full text (Publisher's DOI)
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