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Title
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Varenicline increases in vivo striatal dopamine receptor binding : an ultra-high-resolution pinhole SPECT study in rats
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Author
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Abstract
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| Introduction Ex vivo storage phosphor imaging rat studies reported increased brain dopamine D2/3 receptor (DRD2/3) availability following treatment with varenicline, a nicotinergic drug. However, ex vivo studies can only be performed using cross-sectional designs. Small-animal imaging offers the opportunity to perform serial assessments. We evaluated whether high-resolution pinhole single photon emission computed tomography (SPECT) imaging in rats was able to reproduce previous ex vivo findings. Methods Rats were imaged for baseline striatal DRD2/3 availability using ultra-high-resolution pinhole SPECT (U-SPECT-II) and [123I]IBZM as a radiotracer, and randomized to varenicline (n=7; 2 mg/kg) or saline (n=7). Following 2 weeks of treatment, a second scan was acquired. Results Significantly increased striatal DRD2/3 availability was found following varenicline treatment compared to saline (time⁎treatment effect): posttreatment difference in binding potential between groups corrected for initial baseline differences was 2.039 (P=.022), indicating a large effect size (d=1.48). Conclusions Ultra-high-resolution pinhole SPECT can be used to assess varenicline-induced changes in DRD2/3 availability in small laboratory animals over time. Future small-animal studies should include imaging techniques to enable repeated within-subjects measurements and reduce the amount of animals. |
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Language
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English
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Source (journal)
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Nuclear geophysics. - Oxford, 1993, currens
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Publication
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Oxford
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2012
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ISSN
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0969-8086
[print]
1878-6383
[online]
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DOI
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10.1016/J.NUCMEDBIO.2011.11.006
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Volume/pages
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39
:5
(2012)
, p. 640-644
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Full text (Publisher's DOI)
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Full text (publisher's version - intranet only)
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