Title
Loading of acute myeloid leukemia cells with poly(i:C) by electroporation. Loading of acute myeloid leukemia cells with poly(i:C) by electroporation.
Author
Faculty/Department
Faculty of Medicine and Health Sciences
Publication type
article
Publication
Totowa, N.J. ,
Subject
Human medicine
Source (journal)
Methods in molecular biology. - Totowa, N.J., 1984, currens
Volume/pages
1139(2014) , p. 233-241
ISSN
1064-3745
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Affiliation
University of Antwerp
Abstract
In this chapter, we describe the technique of electroporation as an efficient method to load primary leukemic cells with the double-stranded RNA (dsRNA) analogue, polyriboinosinic polyribocytidylic acid (poly(I:C)), and detail on the delicate freezing and thawing procedure of primary leukemic cells.Electroporation is a non-viral gene transfer method by which short-term pores in the membrane of cells are generated by an electrical pulse, allowing molecules to enter the cell. RNA electroporation, a technique developed in our laboratory, is a widely used and versatile transfection method for efficient introduction of both coding RNA (messenger RNA) and non-coding RNA, e.g., dsRNA and small interfering (siRNA), into mammalian cells. Accurate cell processing and storage of patient material is essential for optimal recovery and quality of the cell product for downstream applications.
Handle