Title
Ammonium improves elution of fixed dried blood spots without affecting immunofluorescence assay quality Ammonium improves elution of fixed dried blood spots without affecting immunofluorescence assay quality
Author
Faculty/Department
Faculty of Sciences. Biology
Publication type
article
Publication
Oxford ,
Subject
Biology
Human medicine
Source (journal)
Tropical medicine and international health. - Oxford
Volume/pages
19(2014) :4 , p. 413-416
ISSN
1360-2276
ISI
000334398000005
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Affiliation
University of Antwerp
Abstract
Objective To solve the problem of fixed dried blood spot elution without damaging IgG antibodies. Methods The minimum effective concentration of liquid ammonium (NH3) in a PBS solution, which was found to elute fixed blood, was determined. By using a dilution series, the effects of NH3 on IgG antibody quality were assessed using immunofluorescence assays. Results The minimum effective concentration of 0.2% NH3 has no detectable effects on IgG quality. Conclusion Ammonium greatly improves blood elution from fixed DBS while maintaining IgG antibody quality. These results are encouraging and provide a basis for further testing of the efficacy of ammonium in different situations as well as its effect on other compounds.
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