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Title
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Ammonium improves elution of fixed dried blood spots without affecting immunofluorescence assay quality
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Author
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Abstract
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| Objective To solve the problem of fixed dried blood spot elution without damaging IgG antibodies. Methods The minimum effective concentration of liquid ammonium (NH3) in a PBS solution, which was found to elute fixed blood, was determined. By using a dilution series, the effects of NH3 on IgG antibody quality were assessed using immunofluorescence assays. Results The minimum effective concentration of 0.2% NH3 has no detectable effects on IgG quality. Conclusion Ammonium greatly improves blood elution from fixed DBS while maintaining IgG antibody quality. These results are encouraging and provide a basis for further testing of the efficacy of ammonium in different situations as well as its effect on other compounds. |
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Language
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English
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Source (journal)
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Tropical medicine and international health. - Oxford
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Publication
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Oxford
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2014
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ISSN
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1360-2276
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DOI
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10.1111/TMI.12259
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Volume/pages
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19
:4
(2014)
, p. 413-416
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ISI
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000334398000005
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Full text (Publisher's DOI)
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Full text (publisher's version - intranet only)
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