Publication
Title
Ammonium improves elution of fixed dried blood spots without affecting immunofluorescence assay quality
Author
Abstract
Objective To solve the problem of fixed dried blood spot elution without damaging IgG antibodies. Methods The minimum effective concentration of liquid ammonium (NH3) in a PBS solution, which was found to elute fixed blood, was determined. By using a dilution series, the effects of NH3 on IgG antibody quality were assessed using immunofluorescence assays. Results The minimum effective concentration of 0.2% NH3 has no detectable effects on IgG quality. Conclusion Ammonium greatly improves blood elution from fixed DBS while maintaining IgG antibody quality. These results are encouraging and provide a basis for further testing of the efficacy of ammonium in different situations as well as its effect on other compounds.
Language
English
Source (journal)
Tropical medicine and international health. - Oxford
Publication
Oxford : 2014
ISSN
1360-2276
Volume/pages
19:4(2014), p. 413-416
ISI
000334398000005
Full text (Publisher's DOI)
Full text (publisher's version - intranet only)
UAntwerpen
Faculty/Department
Research group
Publication type
Subject
Affiliation
Publications with a UAntwerp address
External links
Web of Science
Record
Identification
Creation 31.03.2014
Last edited 07.08.2017
To cite this reference