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Title
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Validation of the AD-CSF-index in autopsy-confirmed Alzheimer's disease patients and healthy controls
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Author
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Abstract
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| The cerebrospinal fluid (CSF) biomarkers amyloid-β peptide of 42 amino acids (Aβ1-42), total tau-protein (T-tau), and tau phosphorylated at threonine 181 (P-tau181P) are used to diagnose Alzheimer's disease (AD). In order to increase diagnostic power, several biomarker combinations have been proposed. In that sense, a new CSF biomarker index was developed, the AD-CSF-index, which has been validated in clinically diagnosed AD patients using electrochemoluminescence based MesoScaleDiscovery and single-analyte ELISA kits. This study validated the AD-CSF-index in neuropathologically diagnosed AD patients, using both single-analyte ELISA and multi-analyte Luminex assays. CSF of 51 neuropathologically diagnosed AD patients and of 95 controls was analyzed by commercially available single-analyte ELISA-kits (INNOTEST®, Innogenetics) and by a Research Use Only version of the multi-analyte Luminex xMAP® assay (INNO-BIA AlzBio3, Innogenetics). Subsequently the AD-CSF-indices were calculated. Both T-tau and P-tau181P AD-CSF-indices were significantly increased in AD patients when compared to controls (p < 0.001). The diagnostic power of the indices was calculated using ROC analyses, resulting in excellent sensitivity and specificity values that systematically exceeded the 80% threshold for discriminating autopsy-confirmed AD patients from controls, independent of the analytical platform. The power to discriminate between AD and non-AD dementias was not included in this study and should be validated in the future. In conclusion, this study validated the AD-CSF index in autopsy-confirmed AD patients and has shown that its excellent diagnostic accuracy is independent of the analytical platform. |
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Language
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English
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Source (journal)
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Journal of Alzheimer's disease. - -
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Publication
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2014
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ISSN
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1387-2877
1875-8908
[Electronic]
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DOI
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10.3233/JAD-131085
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Volume/pages
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41
:3
(2014)
, p. 903-909
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ISI
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000339329100026
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Full text (Publisher's DOI)
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