Title
Determination of trace selenium in biological material by preconcentration and X-ray emission spectrometry Determination of trace selenium in biological material by preconcentration and X-ray emission spectrometry
Author
Faculty/Department
Faculty of Sciences. Chemistry
Faculty of Pharmaceutical, Biomedical and Veterinary Sciences. Pharmacy
Publication type
article
Publication
Amsterdam ,
Subject
Chemistry
Source (journal)
Analytica chimica acta. - Amsterdam
Volume/pages
147(1983) , p. 113-121
ISSN
0003-2670
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Affiliation
University of Antwerp
Abstract
Selenium is determined in the ng g−1 to μg g−1 range in biological and environmental samples. A wet digestion procedure was optimized with respect to volatility losses and recovery yields, by using 75Se metabolically incorporated into rat organs. Selenium is preconcentrated from the digestion liquid by a two-step reduction with 4 M HCl and ascorbic acid. The colloidal selenium formed is adsorbed on activated carbon and filtered on a Nucleopore membrane for measurement by energy-dispersive x-ray fluorescence. Almost complete recovery was obtained, and the detection limit was 20 ng, corresponding to 10 ng g−1 for a 2-g sample. Biological reference materials were analyzed with satisfactory results, and the accuracy of the method was good.
E-info
https://repository.uantwerpen.be/docman/iruaauth/96c308/521a3439679.pdf
Handle