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Title
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Determination of trace selenium in biological material by preconcentration and X-ray emission spectrometry
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Author
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Abstract
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| Selenium is determined in the ng g−1 to μg g−1 range in biological and environmental samples. A wet digestion procedure was optimized with respect to volatility losses and recovery yields, by using 75Se metabolically incorporated into rat organs. Selenium is preconcentrated from the digestion liquid by a two-step reduction with 4 M HCl and ascorbic acid. The colloidal selenium formed is adsorbed on activated carbon and filtered on a Nucleopore membrane for measurement by energy-dispersive x-ray fluorescence. Almost complete recovery was obtained, and the detection limit was 20 ng, corresponding to 10 ng g−1 for a 2-g sample. Biological reference materials were analyzed with satisfactory results, and the accuracy of the method was good. |
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Language
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English
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Source (journal)
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Analytica chimica acta. - Amsterdam, 1947, currens
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Publication
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Amsterdam
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1983
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ISSN
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0003-2670
[print]
1873-4324
[online]
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DOI
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10.1016/0003-2670(83)80077-4
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Volume/pages
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147
(1983)
, p. 113-121
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ISI
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A1983QH42100010
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Full text (Publisher's DOI)
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Full text (publisher's version - intranet only)
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