Title
Laser microprobe mass spectrometry of platinum in dog kidney after cisplatin adminstration
Author
Faculty/Department
Faculty of Sciences. Chemistry
Faculty of Medicine and Health Sciences
Publication type
article
Publication
London ,
Subject
Chemistry
Human medicine
Source (journal)
Biomedical mass spectrometry. - London, 1974 - 1985
Volume/pages
11(1984) :4 , p. 159-163
ISSN
0306-042X
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Affiliation
University of Antwerp
Abstract
By means of laser microprobe mass analysis (LAMMA) platinum was detected in the renal proximal tubular cells of a dog that had been intravenously adminstered the antitumor drug cisplatin (5 mg per kg body weight). No definite subcellular localization of the heavy metal was obtained. Sample prparation and analytical features are examined to increase spatial resolution of analysis while maintaining sufficient detection efficiency. The LAMMA method is destructive, but the amount and type of evaporated material can readily be determined when using LAMMA in combination with transmission electron microscopy. Instrumental optimization and standardization of mass signals is possible by using platinum-loaded, ion chelating resin beads embedded and sectioned with the tissue.
Handle