Title
Improving method reliability in carotenoid analysis through selective removal of glycerolipid interferences by lipase treatment Improving method reliability in carotenoid analysis through selective removal of glycerolipid interferences by lipase treatment
Author
Faculty/Department
Faculty of Pharmaceutical, Biomedical and Veterinary Sciences. Pharmacy
Publication type
article
Publication
Washington, D.C. ,
Subject
Chemistry
Biology
Pharmacology. Therapy
Source (journal)
Journal of agricultural and food chemistry. - Washington, D.C.
Volume/pages
62(2014) :14 , p. 3114-3124
ISSN
0021-8561
ISI
000334572500012
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Affiliation
University of Antwerp
Abstract
Saponification is most often used to hydrolyze glycerolipid interferences during carotenoid analysis. Ester bonds of other plant metabolites such as carotenoids are, however, also hydrolyzed during saponification, thus altering the natural carotenoid composition. A straightforward and selective cleanup procedure was therefore developed involving the enzymatic hydrolysis of matrix glycerolipids. The optimized procedure (100 mu L of extracted vegetable or algal oil in 20 mL of 50:50 phosphate buffer/methanol with 25 mu L of sodium n-octyl sulfate, 30 mg of bile salts, and 250 mu L of NaCl solution (5 mM), magnetic stirring for 2 h at 40 degrees C with 1 mL of Lipozyme TL 100 L and 1 mL of Lipozyme CALB L) removed the greater part of triglycerides (94.8-100.0%) and diglycerides (88.2-99.8%) while preserving the natural carotenoid composition.
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