Title
Flexible nanosomes (SECosomes) enable efficient siRNA delivery in cultured primary skin cells and in the viable epidermis of ex vivo human skin
Author
Faculty/Department
Faculty of Pharmaceutical, Biomedical and Veterinary Sciences . Biomedical Sciences
Publication type
article
Publication
Weinheim ,
Subject
Physics
Human medicine
Source (journal)
Advanced functional materials. - Weinheim
Volume/pages
20(2010) :23 , p. 4077-4090
ISSN
1616-301X
ISI
000285392900006
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Abstract
The extent to which nanoscale-engineered systems cross intact human skin and can exert pharmacological effects in viable epidermis is controversial. This research seeks to develop a new lipid-based nanosome that enables the effective delivery of siRNA into human skin. The major finding is that an ultraflexible siRNA-containing nanosomeprepared using DOTAP, cholesterol, sodium cholate, and 30% ethanolpenetrates into the epidermis of freshly excised intact human skin and is able to enter into the keratinocytes. The nanosomes, called surfactant-ethanol-cholesterol-osomes (SECosomes), show excellent size, surface charge, morphology, deformability, transfection efficiency, stability, and skin penetration capacity after complexation with siRNA. Importantly, these nanosomes have ideal characteristics for siRNA encapsulation, in that the siRNA is stable for at least 4 weeks, they enable highly efficient transfection of in vitro cultured cells, and are shown to transport siRNA delivery through intact human skin where changes in the keratinocyte cell state are demonstrated. It is concluded that increasing flexibility in nanosomes greatly enhances their ability to cross the intact human epidermal membrane and to unload their payload into targeted epidermal cells.
E-info
https://repository.uantwerpen.be/docman/iruaauth/d7471e/6ca37a42125.pdf
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