Title
**Caenorhabditis elegans** nicotinic acetylcholine receptors are required for nociception **Caenorhabditis elegans** nicotinic acetylcholine receptors are required for nociception
Author
Faculty/Department
Faculty of Sciences. Biology
Publication type
article
Publication
Orlando, Fla ,
Subject
Chemistry
Biology
Human medicine
Source (journal)
Molecular and cellular neuroscience. - Orlando, Fla
Volume/pages
59(2014) , p. 85-96
ISSN
1044-7431
ISI
000336338400009
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Affiliation
University of Antwerp
Abstract
Polymodal nociceptors sense and integrate information on injurious mechanical, thermal, and chemical stimuli. Chemical signals either activate nociceptors or modulate their responses to other stimuli. One chemical known to activate or modulate responses of nociceptors is acetylcholine (ACh). Across evolution nociceptors express subunits of the nicotinic acetylcholine receptor (nAChR) family, a family of ACh-gated ion channels. The roles of ACh and nAChRs in nociceptor function are, however, poorly understood. Caenorhabditis elegans polymodal nociceptors, PVD, express nAChR subunits on their sensory arbor. Here we show that mutations reducing ACh synthesis and mutations in nAChR subunits lead to defects in PVD function and morphology. A likely cause for these defects is a reduction in cytosolic calcium measured in ACh and nAChR mutants. Indeed, overexpression of a calcium pump in PVD mimics defects in PVD function and morphology found in nAChR mutants. Our results demonstrate, for the first time, a central role for nAChRs and ACh in nociceptor function and suggest that calcium permeating via nAChRs facilitates activity of several signaling pathways within this neuron.
E-info
https://repository.uantwerpen.be/docman/iruaauth/dec47a/cf25636237e.pdf
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