Title
An electrochemical impedimetric aptasensing platform for sensitive and selective detection of small molecules such as chloramphenicol An electrochemical impedimetric aptasensing platform for sensitive and selective detection of small molecules such as chloramphenicol
Author
Faculty/Department
Faculty of Sciences. Biology
Faculty of Sciences. Chemistry
Faculty of Applied Engineering Sciences
Publication type
article
Publication
Subject
Physics
Chemistry
Biology
Source (journal)
Sensors. - -
Volume/pages
14(2014) :7 , p. 12059-12069
ISSN
1424-8220
ISI
000340035700041
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Affiliation
University of Antwerp
Abstract
We report on the aptadetection of chloramphenicol (CAP) using electrochemical impedance spectroscopy. The detection principle is based on the changes of the interfacial properties of the electrode after the interaction of the ssDNA aptamers with the target molecules. The electrode surface is partially blocked due to the formation of the aptamer-CAP complex, resulting in an increase of the interfacial electron-transfer resistance of the redox probe detected by electrochemical impedance spectroscopy or cyclic voltammetry. We observed that the ratio of polarization resistance had a linear relationship with the concentrations of CAP in the range of 1.76127 nM, and a detection limit of 1.76 nM was obtained. The covalent binding of CAP-aptamer on the electrode surface combined with the unique properties of aptamers and impedimetric transduction leads to the development of a stable and sensitive electrochemical aptasensor for CAP.
Full text (open access)
https://repository.uantwerpen.be/docman/irua/dca3e9/04d14b87.pdf
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