Title
Aptamer-based extraction of ergot alkaloids from ergot contaminated rye feed Aptamer-based extraction of ergot alkaloids from ergot contaminated rye feed
Author
Faculty/Department
Faculty of Sciences. Biology
Faculty of Pharmaceutical, Biomedical and Veterinary Sciences. Pharmacy
Publication type
article
Publication
Irvine, CA :Scientific Research Publishing, Inc ,
Subject
Biology
Pharmacology. Therapy
Source (journal)
Advances in bioscience and biotechnology. - Irvine, CA, 2010, currens
Volume/pages
5(2014) :8 , p. 692-698
ISSN
2156-8456
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Affiliation
University of Antwerp
Abstract
Ergot alkaloids are mycotoxins which can be found in food based on cereal-crops, due to a contamination of plants by fungi of the genus Claviceps. The ingestion of ergot contaminated cereal crops can lead to a severe poisoning known as ergotism. For food and feed safety purposes, the extraction of ergot alkaloids from ergot contaminated flour was investigated. For the specific recognition of ergot alkaloids, DNA aptamer ligands specially selected for ergot alkaloids were grafted onto silica gel in order to construct a specific solid phase extraction system. The aptamer-functionalized silica gels were used to extract ergot alkaloids from a contaminated rye feed sample. The presence of ergot alkaloids eluted from the aptamer-functionalized silica gels was analyzed using LC-QTOF-MS. By using this simple system, it was possible to specifically extract ergosine, ergokryptine and ergocornine from an ergot contaminated rye feed sample. This aptamer-based extraction tool shows the applicability of aptamers for the specific extraction of toxins or natural compounds from turbid matrices in a one-step procedure.
Full text (open access)
https://repository.uantwerpen.be/docman/irua/d216ed/4002ffcd.pdf
Handle