Title
Sperm involved in recurrent partial hydatidiform moles cannot induce the normal pattern of calcium oscillations Sperm involved in recurrent partial hydatidiform moles cannot induce the normal pattern of calcium oscillations
Author
Faculty/Department
Faculty of Pharmaceutical, Biomedical and Veterinary Sciences . Biomedical Sciences
Publication type
article
Publication
Baltimore, Md ,
Subject
Biology
Human medicine
Source (journal)
Fertility and sterility. - Baltimore, Md
Volume/pages
102(2014) :2 , p. 581-U337
ISSN
0015-0282
ISI
000341299000042
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Affiliation
University of Antwerp
Abstract
Objective: To assess the Ca2þ-releasing ability of sperm involved in partial hydatidiform moles. Design: Analysis of the activating and Ca2þ-releasing ability of human sperm. Setting: University hospital research laboratory. Patient(s): Patients undergoing intracytoplasmic sperm injection (ICSI) treatment. Intervention(s): Microinjection of mouse and human oocytes with sperm. Main Outcome Measure(s): Measurement of the fertilizing and Ca2þ-releasing ability of human sperm. Result(s): The mouse oocyte Ca2þ analysis showed that only 19.0% (4/21) of the mouse oocytes injected with sperm involved in molar pregnancies exhibited a normal pattern of Ca2þ oscillations versus 63.2% (36/57) of those injected with control sperm. Further, 83.3% (15/18) of donated in vitromatured human oocytes injected with deficient sperm did not exhibit any Ca2þ release, while 76.9% (10/13) failed to show normal pronuclear development. Yet the sperm oocyte activation factor phospholipase C zeta (PLCz) was present in the majority (96.6%, n ¼ 113) of the analyzed sperm at a normal expression level. Eventually, fertilization failure was overcome with assisted oocyte activation in subsequent therapeutic ICSI cycles, which led to normal deliveries. Conclusion(s): Sperm that previously provoked recurrent partial hydatidiform mole pregnancies due to dispermic fertilization is not able to activate human oocytes or trigger the normal pattern of Ca2þ oscillations in mouse and human oocytes in vitro. (Fertil Steril 2014;102: 5818. 2014 by American Society for Reproductive Medicine.)
E-info
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