Publication
Title
Significance of bacterial surface molecules interactions with milk proteins to enhance microencapsulation of **Lactobacillus rhamnosus** GG
Author
Abstract
Probiotic bacteria are being increasingly encapsulated to enhance their delivery in an active state at their preferred site of action. In this study, an encapsulation process based on emulsification and requiring only food grade components was used to protect wild-type Lactobacillus rhamnosus GG (LGG) and three of its surface mutants into dairy matrices. The mechanism of microencapsulation was studied at the molecular level by comparing the encapsulation efficiency of LGG wild type and three of its surface mutants with Atomic Force Microscopy. A significant decrease in the encapsulation efficiency was observed when the bacteria were depleted for pili, while the pilus also appeared to be crucial for location of LGG inside the microparticle. Hereto, the spaCBA mutant lacking pili), the welE mutant lacking long exopolysaccharides) and the dltD mutant having modified lipoteichoic acids were used. Atomic Force Microscopy enabled the confirmation of specific interactions between bacteria and whey proteins, in contrast to the observed nonspecific interactions with rnicellar casein. The role of the pili, i.e. multimeric appendages of several micrometers, was also modeled using WLC (Worm-Like Chain) or FJC (Freely Jointed Chain) models. This revealed that understanding molecular mechanisms of microencapsulation of probiotic bacteria should ultimately benefit their targeted application. (C) 2014 Elsevier Ltd. All rights reserved.
Language
English
Source (journal)
Food hydrocolloids. - Oxford
Publication
Oxford : 2014
ISSN
0268-005X
Volume/pages
41(2014), p. 60-70
ISI
000338004700008
Full text (Publisher's DOI)
Full text (publisher's version - intranet only)
UAntwerpen
Faculty/Department
Research group
Publication type
Subject
Affiliation
Publications with a UAntwerp address
External links
Web of Science
Record
Identification
Creation 28.08.2014
Last edited 12.06.2017
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