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Title
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Automated analytical standard production with supercritical fluid chromatography for the quantification of bioactive C17-polyacetylenes : a case study on food processing waste
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Author
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Abstract
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| Food processing enterprises produce enormous amounts of organic waste that contains valuable phytochemicals (e.g. C17-polyacetylenes). Knowledge on the phytochemicals content is a first step towards valorisation. Quantification of C17-polyacetylenes is however often hampered by the lack of commercially available standards or by tedious multistep in-house standard production procedures. In the current study, a new and straightforward supercritical fluid chromatography purification procedure is described for the simultaneous production of 2 analytical C17-polyacetylene standards. Respectively, 5 and 6 mg of falcarinol and falcarindiol were purified in 17 h on analytical scale. After confirming the identity and quality (97% purity) by Nuclear Magnetic Resonance, accurate mass-Mass Spectrometry (am-MS) and Photo Diode Array (PDA) detection the C17-polyacetylene standards were used for the analysis of industrial vegetable waste with Liquid Chromatography coupled to PDA and am-MS detection. Measurements showed varying concentrations of C17-polyacetylenes in the organic waste depending on its nature and origin. (C) 2014 Elsevier Ltd. All rights reserved. |
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Language
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English
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Source (journal)
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Food chemistry. - Amsterdam
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Publication
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Amsterdam
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2014
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ISSN
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0308-8146
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DOI
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10.1016/J.FOODCHEM.2014.05.093
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Volume/pages
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165
(2014)
, p. 371-378
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ISI
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000340303600049
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Full text (Publisher's DOI)
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Full text (publisher's version - intranet only)
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