Title
Small-animal repetitive transcranial magnetic stimulation combined with [<tex>$^{18}F$</tex>]-FDG micropet to quantify the neuromodulation effect in the rat brain Small-animal repetitive transcranial magnetic stimulation combined with [<tex>$^{18}F$</tex>]-FDG micropet to quantify the neuromodulation effect in the rat brain
Author
Faculty/Department
Faculty of Medicine and Health Sciences
Publication type
article
Publication
Oxford ,
Subject
Human medicine
Source (journal)
Neuroscience / International Brain Research Organization. - Oxford
Volume/pages
275(2014) , p. 436-443
ISSN
0306-4522
ISI
000340083500039
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Affiliation
University of Antwerp
Abstract
Repetitive transcranial magnetic stimulation (rTMS) is a non-invasive neurostimulation technique for the treatment of various neurological and psychiatric disorders. To investigate the working mechanism of this treatment approach, we designed a small-animal coil for dedicated use in rats and we combined this neurostimulation method with small-animal positron emission tomography (microPET or mu PET) to quantify regional 2-deoxy-2-(F-18)fluoro-D-glucose ([F-18]-FDG) uptake in the rat brain, elicited by a low- (1 Hz) and a high- (50 Hz) frequency paradigm. Rats (n = 6) were injected with 1 mCi of [F-18]-FDG 10 min after the start of 30 min of stimulation (1 Hz, 50 Hz or sham), followed by a 20-min mu PET image acquisition. Voxel-based statistical parametric mapping (SPM) image analysis of 1-Hz and 50-Hz versus sham stimulation was performed. For both the 1-Hz and 50-Hz paradigms we found a large [F-18]-FDG hypermetabolic cluster (2.208 mm(3) and 2.616 mm(3), resp.) (analysis of variance (ANOVA), p < 0.05) located in the dentate gyrus complemented with an additional [F-18]-FDG hypermetabolic cluster (ANOVA, p < 0.05) located in the entorhinal cortex (2.216 mm(3)) for the 50-Hz stimulation. The effect on [F-18]-FDG metabolism was 2.9 +/- 0.8% at 1 Hz and 2.5 +/- 0.8% at 50 Hz for the dentate gyrus clusters and 3.3 +/- 0.5% for the additional cluster in the entorhinal cortex at 50 Hz. The maximal (4.19 vs. 2.58) and averaged (2.87 vs. 2.21) T-values are higher for 50 Hz versus 1 Hz. This experimental study demonstrates the feasibility to combine mu PET imaging in rats stimulated with rTMS using a custom-made small-animal magnetic stimulation setup to quantify changes in the cerebral [F-18]-FDG uptake as a measure for neuronal activity. (C) 2014 IBRO. Published by Elsevier Ltd. All rights reserved.
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