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Title
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Flow cytometric allergy diagnosis : basophil activation techniques
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Author
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Abstract
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| The basis of flow cytometric allergy diagnosis is quantification of changes in expression of basophilic surface membrane markers (Ebo et al., Clin Exp Allergy 34: 332339, 2004). Upon encountering specific allergens recognized by surface receptor FcεRI-bound IgE, basophils not only secrete and generate quantifiable bioactive mediators but also up-regulate the expression of different markers (e.g., CD63, CD203c) which can be detected by multicolor flow cytometry using specific monoclonal antibodies (Ebo et al., Cytometry B Clin Cytom 74: 201210, 2008). Here, we describe two flow cytometry-based protocols which allow detection of surface marker activation (Method 1) and changes in intragranular histamine (Method 2), both reflecting different facets of basophil activation. |
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Language
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English
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Source (book)
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Basophils and mast cells : methods and protocols / Gibbs, Bernard F. [edit.]; et al.
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Source (series)
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Methods in molecular biology ; 1192
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Publication
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New York, N.Y.
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Springer
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2014
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ISBN
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978-1-4939-1172-1
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DOI
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10.1007/978-1-4939-1173-8_11
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Volume/pages
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p. 147-159
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Full text (Publisher's DOI)
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