Publication
Title
Flow cytometric allergy diagnosis : basophil activation techniques
Author
Abstract
The basis of flow cytometric allergy diagnosis is quantification of changes in expression of basophilic surface membrane markers (Ebo et al., Clin Exp Allergy 34: 332339, 2004). Upon encountering specific allergens recognized by surface receptor FcεRI-bound IgE, basophils not only secrete and generate quantifiable bioactive mediators but also up-regulate the expression of different markers (e.g., CD63, CD203c) which can be detected by multicolor flow cytometry using specific monoclonal antibodies (Ebo et al., Cytometry B Clin Cytom 74: 201210, 2008). Here, we describe two flow cytometry-based protocols which allow detection of surface marker activation (Method 1) and changes in intragranular histamine (Method 2), both reflecting different facets of basophil activation.
Language
English
Source (book)
Basophils and mast cells : methods and protocols / Gibbs, Bernard F. [edit.]; et al.
Source (series)
Methods in molecular biology ; 1192
Publication
New York, N.Y. : Springer, 2014
ISBN
978-1-4939-1172-1
Volume/pages
p. 147-159
Full text (Publisher's DOI)
UAntwerpen
Faculty/Department
Research group
[E?say:metaLocaldata.cgzprojectinf]
Publication type
Subject
Affiliation
Publications with a UAntwerp address
External links
Record
Identification
Creation 17.09.2014
Last edited 22.11.2016
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