Title
Flow cytometric allergy diagnosis : basophil activation techniques Flow cytometric allergy diagnosis : basophil activation techniques
Author
Faculty/Department
Faculty of Medicine and Health Sciences
Publication type
bookPart
Publication
New York, N.Y. :Springer, [*]
Subject
Human medicine
Source (book)
Basophils and mast cells : methods and protocols / Gibbs, Bernard F. [edit.]; et al.
Source (series)
Methods in molecular biology ; 1192
ISBN - Hoofdstuk
978-1-4939-1172-1
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Affiliation
University of Antwerp
Abstract
The basis of flow cytometric allergy diagnosis is quantification of changes in expression of basophilic surface membrane markers (Ebo et al., Clin Exp Allergy 34: 332339, 2004). Upon encountering specific allergens recognized by surface receptor FcεRI-bound IgE, basophils not only secrete and generate quantifiable bioactive mediators but also up-regulate the expression of different markers (e.g., CD63, CD203c) which can be detected by multicolor flow cytometry using specific monoclonal antibodies (Ebo et al., Cytometry B Clin Cytom 74: 201210, 2008). Here, we describe two flow cytometry-based protocols which allow detection of surface marker activation (Method 1) and changes in intragranular histamine (Method 2), both reflecting different facets of basophil activation.
Handle