Title
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The skin microbiome of caspase-14-deficient mice shows mild dysbiosis
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Author
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Abstract
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Caspase-14, an important proteinase involved in filaggrin catabolism, is mainly active in terminally differentiating keratinocytes, where it is required for the generation of skin natural moisturizing factors (NMFs). Consequently, caspase-14 deficient epidermis is characterized by reduced levels of NMFs such as urocanic acid and 2-pyrrolidone-5-carboxylic acid. Patients suffering from filaggrin deficiency are prone to develop atopic dermatitis, which is accompanied with increased microbial burden. Among several reasons, this effect could be due to a decrease in filaggrin breakdown products. In this study, we found that caspase-14(-/-) mice show enhanced antibacterial response compared to wild-type mice when challenged with bacteria. Therefore, we compared the microbial communities between wild-type and caspase-14(-/-) mice by sequencing of bacterial 16S ribosomal RNA genes. We observed that caspase-14 ablation leads to an increase in bacterial richness and diversity during steadystate conditions. Although both wild-type and caspase-14(-/-) skin were dominated by the Firmicutes phylum, the Staphylococcaceae family was reduced in caspase-14(-/-) mice. Altogether, our data demonstrated that caspase-14 deficiency causes the imbalance of the skin-resident bacterial communities. |
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Language
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English
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Source (journal)
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Experimental dermatology. - Copenhagen
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Publication
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Copenhagen
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2014
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ISSN
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0906-6705
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DOI
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10.1111/EXD.12458
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Volume/pages
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23
:8
(2014)
, p. 561-567
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ISI
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000340536100010
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Full text (Publisher's DOI)
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Full text (publisher's version - intranet only)
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