Publication
Title
A generic approach for shotgun analysis of the soluble proteome of plant cell suspension cultures
Author
Abstract
Cell suspension cultures from different plant species act as important model systems for studying cellular processes in plant biology and are often used as green factories for the production of valuable secondary metabolites and recombinant proteins. While mass spectrometry based proteome analysis techniques are ideally suited to study plant cell metabolism and other fundamental cellular processes from a birds eye perspective, they remain underused in plant studies. We describe a comprehensive sample preparation and multidimensional shotgun proteomics strategy that can be generically applied to plant cell suspension cultures. This strategy was optimized and tested on an Arabidopsis thaliana ecotype Landsberg erecta culture. Furthermore, the implementation of strong cation exchange chromatography as a peptide fractionation step is elaborately tested. Its utility in mass spectrometry based proteome analysis is discussed. Using the presented analytical platform, over 13,000 unique peptides and 2640 proteins could be identified from a single plant cell suspension sample. Finally, the experimental setup is validated using Nicotiana tabacum cv. Bright Yellow-2 (BY-2) plant cell suspension cultures, thereby demonstrating that the presented analytical platform can also be valuable tool in proteome analysis of non-genomic model systems.
Language
English
Source (journal)
Journal of chromatography : B: analytical technologies in the biomedical and life sciences. - Amsterdam, 2002, currens
Publication
Amsterdam : 2015
ISSN
1570-0232 [print]
1873-376X [online]
Volume/pages
974(2015), p. 48-56
ISI
000346540800007
Full text (Publishers DOI)
Full text (open access)
UAntwerpen
Faculty/Department
Research group
Publication type
Subject
Affiliation
Publications with a UAntwerp address
External links
Web of Science
Record
Identification
Creation 31.10.2014
Last edited 26.03.2017
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