Involvement of inward rectifier and M-type currents in carbachol-induced epileptiform synchronization
Faculty of Pharmaceutical, Biomedical and Veterinary Sciences . Biomedical Sciences
Neuropharmacology. - Oxford, 1970, currens
, p. 653-661
Exposure to cholinergic agonists is a widely used paradigm to induce epileptogenesis in vivo and synchronous activity in brain slices maintained in vitro. However, the mechanisms underlying these effects remain unclear. Here, we used field potential recordings from the lateral entorhinal cortex in horizontal rat brain slices to explore whether two different K+ currents regulated by muscarinic receptor activation, the inward rectifier (KIR) and the M-type (KM) currents, have a role in carbachol (CCh)-induced field activity, a prototypical model of cholinergic-dependent epileptiform synchronization. To establish whether KIR or KM blockade could replicate CCh effects, we exposed slices to blockers of these currents in the absence of CCh. KIR channel blockade with micromolar Ba2+ concentrations induced interictal-like events with duration and frequency that were lower than those observed with CCh; by contrast, the KM blocker linopirdine was ineffective. Pre-treatment with Ba2+ or linopirdine increased the duration of epileptiform discharges induced by subsequent application of CCh. Baclofen, a GABAB receptor agonist that activates KIR, abolished CCh-induced field oscillations, an effect that was abrogated by the GABAB receptor antagonist CGP 55845, and prevented by Ba2+. Finally, when applied after CCh, the KM activators flupirtine and retigabine shifted leftward the cumulative distribution of CCh-induced event duration; this effect was opposite to what seen during linopirdine application under similar experimental conditions. Overall, our findings suggest that KIR rather than KM plays a major regulatory role in controlling CCh-induced epileptiform synchronization.