Publication
Title
Differential effects of linoleic and alpha-linolenic fatty acids on spatial and temporal mitochondrial distribution and activity in bovine oocytes
Author
Abstract
Using specific stains and confocal microscope imaging, the patterns of mitochondrial distribution, mitochondrial inner membrane potential and reactive oxygen species (ROS) levels during bovine oocyte maturation were determined in the presence or absence of physiological concentrations of linoleic acid (LA; 100 µM) or α-linolenic acid (ALA; 50 µM). Mitochondrial distribution in control oocytes at 0 h was mainly peripheral and changed to a diffused pattern after 1 h of culture; this was maintained up to 24 h. Mitochondrial clusters were observed during the early hours of maturation (14 h); the majority of these were arranged in perinuclear fashion. LA supplementation resulted in: (1) delayed redistribution of the mitochondria from a peripheral to a diffuse pattern and a decreased percentages of oocytes showing perinuclear mitochondrial clusters, (2) decreased mitochondrial inner membrane potential at 1 and 24 h compared with the control and (3) higher ROS levels, associated with a lower nuclear maturation rate. In contrast, ALA supplementation had no effect on mitochondrial distribution and activity and decreased ROS levels compared with the control; this was associated with an increased nuclear maturation rate. In conclusion, LA induced alterations in mitochondrial distribution and activity as well as increasing ROS levels, which mediate, at least in part, the inhibitory effect on oocyte maturation.
Language
English
Source (journal)
Reproduction, fertility and development. - East Melbourne
Publication
East Melbourne : 2012
ISSN
1031-3613
Volume/pages
24:5(2012), p. 679-690
ISI
000304603300005
Full text (Publisher's DOI)
UAntwerpen
Faculty/Department
Project info
Publication type
Subject
External links
Web of Science
Record
Identification
Creation 03.11.2014
Last edited 14.12.2017