Title
Basophil activation tests : time for a reconsideration Basophil activation tests : time for a reconsideration
Author
Faculty/Department
Faculty of Medicine and Health Sciences
Publication type
article
Publication
London :Expert reviews ,
Subject
Human medicine
Source (journal)
Expert review of clinical immunology
Volume/pages
10(2014) :10 , p. 1325-1335
ISSN
1744-666X
ISI
000342235400007
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Affiliation
University of Antwerp
Abstract
Challenges in in vitro allergy diagnostics lie in the development of accessible and reliable assays allowing identification of all offending allergens and cross-reactive structures. Flow-assisted analysis and quantification of in vitro activated basophils serves as a diagnostic instrument with increasing applications developed over the years. From the earliest days it was clear that the test could constitute a diagnostic asset in basophil-mediated hypersensitivity. However, utility of the basophil activation test should be reassessed regarding difficulties with preparation, characterization and validation of allergen extracts; availability and the potential of more accessible diagnostics. Today, the added value mainly lies in diagnosis of immediate drug hypersensitivity. Other potential indications are monitoring venom-immunotherapy and follow-up of natural history of food allergies. However, results in these nondiagnostic applications are preliminary. We review the most relevant clinical applications of the basophil activation test. Some personal comments and views about perspectives and challenges about flow-assisted allergy diagnosis are made.
Full text (open access)
https://repository.uantwerpen.be/docman/irua/000895/8725.pdf
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