Publication
Title
Isolation and identification of 2 isomeric trihydroxy octadecenoic acids with prostaglandin E-like activity from onion bulbs (**Allium cepa**)
Author
Abstract
Two fractions with prostaglandin E-like activity were isolated from onion () by using XAD-2 adsorption, silicic acid column chromatography and thin layer chromatography. The fractions were analyzed by gas chromatography/mass spectrometry and were characterized as isomeric mixtures of 9,10,13-trihydroxy-11-octadecenoic and 9,12,13-trihydroxy-10-octadecenoic acid, which are lipoxygenase metabolites of linoleic acid. Bio-assay, for which cascade superfusion was used and the rabbit coeliac and mesenteric arteries and the rat fundus strip were employed as assay organs, was utilized to monitor the bio-active profile throughout the isolation procedures. The activity of 1 μg of the pharmacologically active fractions T1 and T2 was found to be equivalent to that of respectively 1.33 and 0.63 ng of prostaglandin E2.
Language
English
Source (journal)
Prostaglandins. - Los Altos, Calif.
Publication
Los Altos, Calif. : 1985
ISSN
0090-6980
Volume/pages
29:5(1985), p. 847-865
ISI
A1985AKA6300015
Full text (Publisher's DOI)
Full text (publisher's version - intranet only)
UAntwerpen
Faculty/Department
Research group
[E?say:metaLocaldata.cgzprojectinf]
Publication type
Subject
Affiliation
Publications with a UAntwerp address
External links
Web of Science
Record
Identification
Creation 18.11.2014
Last edited 10.09.2017
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