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Title
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Isolation and identification of 2 isomeric trihydroxy octadecenoic acids with prostaglandin E-like activity from onion bulbs (**Allium cepa**)
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Author
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Abstract
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| Two fractions with prostaglandin E-like activity were isolated from onion () by using XAD-2 adsorption, silicic acid column chromatography and thin layer chromatography. The fractions were analyzed by gas chromatography/mass spectrometry and were characterized as isomeric mixtures of 9,10,13-trihydroxy-11-octadecenoic and 9,12,13-trihydroxy-10-octadecenoic acid, which are lipoxygenase metabolites of linoleic acid. Bio-assay, for which cascade superfusion was used and the rabbit coeliac and mesenteric arteries and the rat fundus strip were employed as assay organs, was utilized to monitor the bio-active profile throughout the isolation procedures. The activity of 1 μg of the pharmacologically active fractions T1 and T2 was found to be equivalent to that of respectively 1.33 and 0.63 ng of prostaglandin E2. |
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Language
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English
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Source (journal)
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Prostaglandins. - Los Altos, Calif.
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Publication
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Los Altos, Calif.
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1985
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ISSN
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0090-6980
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DOI
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10.1016/0090-6980(85)90142-X
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Volume/pages
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29
:5
(1985)
, p. 847-865
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ISI
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A1985AKA6300015
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Full text (Publisher's DOI)
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Full text (publisher's version - intranet only)
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