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Title
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Artemisinins, new miconazole potentiators resulting in increased activity against Candida albicans biofilms
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Author
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Abstract
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| Mucosal biofilm-related fungal infections are very common and the incidence of recurrent oral and vulvovaginal candidiasis is significant. As resistance against azoles (preferred treatment) is occurring, we aimed at identifying compounds that increase the activity of miconazole against Candida albicans biofilms. We screened 1600 compounds of a drug repositioning library in combination with a sub-inhibitory concentration of miconazole. Synergy between the best identified potentiators and miconazole was characterized by checkerboard analyses and fractional inhibitory concentration indices. Hexachlorophene, pyrvinium pamoate and artesunate act synergistically with miconazole in affecting C. albicans biofilms. Synergy was most pronounced for artesunate and structural homologues thereof. No synergistic effect could be observed between artesunate and fluconazole, caspofungin or amphotericin B. Our data reveal enhancement of the antibiofilm activity of miconazole by artesunate, pointing to potential combination therapy consisting of miconazole and artesunate to treat C. albicans biofilm-related infections. |
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Language
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English
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Source (journal)
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Antimicrobial agents and chemotherapy. - Washington, D.C., 1972, currens
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Publication
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Washington, D.C.
:
American Society for Microbiology
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2015
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ISSN
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0066-4804
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DOI
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10.1128/AAC.04229-14
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Volume/pages
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59
:1
(2015)
, p. 421-426
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ISI
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000348609500051
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Pubmed ID
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25367916
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Full text (Publisher's DOI)
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Full text (open access)
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