Title
3D culture of murine neural stem cells on decellularized mouse brain sections 3D culture of murine neural stem cells on decellularized mouse brain sections
Author
Faculty/Department
Faculty of Medicine and Health Sciences
Publication type
article
Publication
Guildford ,
Subject
Physics
Human medicine
Source (journal)
Biomaterials. - Guildford
Volume/pages
41(2015) , p. 122-131
ISSN
0142-9612
ISI
000349062100012
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Affiliation
University of Antwerp
Abstract
Transplantation of neural stem cells (NSC) in diseased or injured brain tissue is widely studied as a potential treatment for various neurological pathologies. However, effective cell replacement therapy relies on the intrinsic capacity of cellular grafts to overcome hypoxic and/or immunological barriers after transplantation. In this context, it is hypothesized that structural support for grafted NSC will be of utmost importance. With this study, we present a novel decellularization protocol for 1.5 mm thick mouse brain sections, resulting in the generation of acellular three-dimensional (3D) brain sections. Next, the obtained 3D brain sections were seeded with murine NSC expressing both the eGFP and luciferase reporter proteins (NSC-eGFP/Luc). Using real-time bioluminescence imaging, the survival and growth of seeded NSC-eGFP/Luc cells was longitudinally monitored for 17 weeks in culture, indicating the ability of the acellular brain sections to support sustained ex vivo growth of NSC. Next, the organization of a 3D maze-like cellular structure was examined using confocal microscopy. Moreover, under mitogenic stimuli (EGF and hFGF-2), most cells in this 3D culture retained their NSC phenotype. Concluding, we here present a novel protocol for decellularization of mouse brain sections, which subsequently support long-term 3D culture of undifferentiated NSC.
E-info
https://repository.uantwerpen.be/docman/iruaauth/48d7f3/78e2ef032c5.pdf
Full text (open access)
https://repository.uantwerpen.be/docman/irua/a1344e/9282.pdf
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