Title
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3D culture of murine neural stem cells on decellularized mouse brain sections
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Author
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Abstract
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Transplantation of neural stem cells (NSC) in diseased or injured brain tissue is widely studied as a potential treatment for various neurological pathologies. However, effective cell replacement therapy relies on the intrinsic capacity of cellular grafts to overcome hypoxic and/or immunological barriers after transplantation. In this context, it is hypothesized that structural support for grafted NSC will be of utmost importance. With this study, we present a novel decellularization protocol for 1.5 mm thick mouse brain sections, resulting in the generation of acellular three-dimensional (3D) brain sections. Next, the obtained 3D brain sections were seeded with murine NSC expressing both the eGFP and luciferase reporter proteins (NSC-eGFP/Luc). Using real-time bioluminescence imaging, the survival and growth of seeded NSC-eGFP/Luc cells was longitudinally monitored for 17 weeks in culture, indicating the ability of the acellular brain sections to support sustained ex vivo growth of NSC. Next, the organization of a 3D maze-like cellular structure was examined using confocal microscopy. Moreover, under mitogenic stimuli (EGF and hFGF-2), most cells in this 3D culture retained their NSC phenotype. Concluding, we here present a novel protocol for decellularization of mouse brain sections, which subsequently support long-term 3D culture of undifferentiated NSC. |
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Language
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English
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Source (journal)
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Biomaterials. - Guildford
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Publication
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Guildford
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2015
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ISSN
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0142-9612
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DOI
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10.1016/J.BIOMATERIALS.2014.11.025
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Volume/pages
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41
(2015)
, p. 122-131
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ISI
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000349062100012
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Full text (Publisher's DOI)
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Full text (open access)
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Full text (publisher's version - intranet only)
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