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Title
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Rapid and enhanced proteolytic digestion using electric-field-oriented enzyme reactor
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Author
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Abstract
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| We have created a novel enzyme reactor using electric field-mediated orientation and immobilization of proteolytic enzymes (trypsin/chymotrypsin) on biocompatible PVDF membranes in a continuous flow-through chamber. Using less than 5 min, this reactor in various enzyme combinations can produce enhanced rapid digestion for standardized prototypic proteins, hydrophilic proteins and hydrophobic transmembrane proteins when compared to in-solution techniques. With improved digestive efficiency, our reactor improved the overall functional analysis of lipid raft proteomes by identifying more closely functionally linked proteins and elucidated a richer set of biological processes and pathways linked to the proteins,than traditional in-solution methods. Published by Elsevier B.V. |
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Language
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English
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Source (journal)
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Journal of proteomics
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Publication
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2011
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ISSN
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1874-3919
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DOI
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10.1016/J.JPROT.2011.02.007
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Volume/pages
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74
:7
(2011)
, p. 1030-1035
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ISI
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000292440400014
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Full text (Publisher's DOI)
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Full text (publisher's version - intranet only)
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