Publication
Title
Rapid and enhanced proteolytic digestion using electric-field-oriented enzyme reactor
Author
Abstract
We have created a novel enzyme reactor using electric field-mediated orientation and immobilization of proteolytic enzymes (trypsin/chymotrypsin) on biocompatible PVDF membranes in a continuous flow-through chamber. Using less than 5 min, this reactor in various enzyme combinations can produce enhanced rapid digestion for standardized prototypic proteins, hydrophilic proteins and hydrophobic transmembrane proteins when compared to in-solution techniques. With improved digestive efficiency, our reactor improved the overall functional analysis of lipid raft proteomes by identifying more closely functionally linked proteins and elucidated a richer set of biological processes and pathways linked to the proteins,than traditional in-solution methods. Published by Elsevier B.V.
Language
English
Source (journal)
Journal of proteomics
Publication
2011
ISSN
1874-3919
Volume/pages
74:7(2011), p. 1030-1035
ISI
000292440400014
Full text (Publisher's DOI)
Full text (publisher's version - intranet only)
UAntwerpen
Faculty/Department
Project info
Publication type
Subject
External links
Web of Science
Record
Identification
Creation 06.01.2015
Last edited 02.12.2017