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Title
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Isolation and infusion of donor CD34(+) bone marrow cells in cadaver kidney transplantation
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Author
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Abstract
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| Background, Infusion of donor bone marrow cells induces tolerance in allograft models. CD34(+) stem cells present in human bone marrow could be endowed with tolerogenic properties. Methods, CD34(+) stem cells were isolated from bone marrow extracted from vertebral bodies of cadaveric donors. Donor CD34(+) cells (0.6-3.7 x 10(6)/kg) were infused during surgery in 10 kidney transplant recipients receiving OKT3 as induction therapy. Chimerism was investigated using nested PCR for donor-specific HLA alleles. Results. The infusion of CD34(+) stem cells was perfectly tolerated. Five patients remained free of acute rejection at follow-up, 47-325 days post-operatively. The five other patients underwent a single episode of corticosensitive acute rejection. Long-term chimerism was not induced in the seven patients investigated for the persistence of donor DNA. Conclusions, Infusion of donor CD34(+) stem cells in kidney transplantation is safe. The clinical usefulness of the procedure remains to be established. |
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Language
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English
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Source (journal)
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Nephrology, dialysis, transplantation. - Berlin
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Publication
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Berlin
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1998
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ISSN
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0931-0509
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DOI
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10.1093/NDT/13.1.34
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Volume/pages
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13
:1
(1998)
, p. 34-36
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ISI
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000071659200012
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Full text (Publisher's DOI)
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