Title
Isolation and infusion of donor CD34(+) bone marrow cells in cadaver kidney transplantation Isolation and infusion of donor CD34(+) bone marrow cells in cadaver kidney transplantation
Author
Faculty/Department
Faculty of Medicine and Health Sciences
Publication type
article
Publication
Berlin ,
Subject
Human medicine
Source (journal)
Nephrology, dialysis, transplantation. - Berlin
Volume/pages
13(1998) :1 , p. 34-36
ISSN
0931-0509
ISI
000071659200012
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Abstract
Background, Infusion of donor bone marrow cells induces tolerance in allograft models. CD34(+) stem cells present in human bone marrow could be endowed with tolerogenic properties. Methods, CD34(+) stem cells were isolated from bone marrow extracted from vertebral bodies of cadaveric donors. Donor CD34(+) cells (0.6-3.7 x 10(6)/kg) were infused during surgery in 10 kidney transplant recipients receiving OKT3 as induction therapy. Chimerism was investigated using nested PCR for donor-specific HLA alleles. Results. The infusion of CD34(+) stem cells was perfectly tolerated. Five patients remained free of acute rejection at follow-up, 47-325 days post-operatively. The five other patients underwent a single episode of corticosensitive acute rejection. Long-term chimerism was not induced in the seven patients investigated for the persistence of donor DNA. Conclusions, Infusion of donor CD34(+) stem cells in kidney transplantation is safe. The clinical usefulness of the procedure remains to be established.
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