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Title
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Synthesis of a heparin-related GlcN-IdoA sulfation-site variable disaccharide library and analysis by Raman and ROA spectroscopy
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Author
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Abstract
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| Synthesis of an array of differentially sulfated GlcN-IdoA disaccharides, accessible on good scale, directly from L-iduronate components is described. These are specifically directed to provide the sulfation variability at the key most common biologically relevant sulfation-variable L-IdoA O-2 and D-GlcN O-6 and amino sites of this heparin disaccharide. This sulfation-varied matrix has allowed the first evaluation of using Raman/ROA spectroscopy to characterize changes in spectra as a function of both site and level of sulfation with pure, defined heparin-related disaccharide species. This provides analysis of both similarities and differences to digest native heparin and this shows evidence of different types of changes in conformations and conformational freedom as a function of some specific sulfation changes at the disaccharide level. It is anticipated that this data set will open the way for applications to further site-specific sulfated saccharides and demonstrates the capability offered by Raman-ROA towards fingerprinting sulfation in heparin fragments. (C) 2014 The Authors. Published by Elsevier Ltd. |
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Language
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English
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Source (journal)
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Carbohydrate research. - Amsterdam
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Publication
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Amsterdam
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2014
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ISSN
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0008-6215
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DOI
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10.1016/J.CARRES.2014.06.026
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Volume/pages
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400
(2014)
, p. 44-53
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ISI
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000344880900007
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Pubmed ID
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25457609
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Full text (Publisher's DOI)
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Full text (publisher's version - intranet only)
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