Publication
Title
Synthesis of a heparin-related GlcN-IdoA sulfation-site variable disaccharide library and analysis by Raman and ROA spectroscopy
Author
Abstract
Synthesis of an array of differentially sulfated GlcN-IdoA disaccharides, accessible on good scale, directly from L-iduronate components is described. These are specifically directed to provide the sulfation variability at the key most common biologically relevant sulfation-variable L-IdoA O-2 and D-GlcN O-6 and amino sites of this heparin disaccharide. This sulfation-varied matrix has allowed the first evaluation of using Raman/ROA spectroscopy to characterize changes in spectra as a function of both site and level of sulfation with pure, defined heparin-related disaccharide species. This provides analysis of both similarities and differences to digest native heparin and this shows evidence of different types of changes in conformations and conformational freedom as a function of some specific sulfation changes at the disaccharide level. It is anticipated that this data set will open the way for applications to further site-specific sulfated saccharides and demonstrates the capability offered by Raman-ROA towards fingerprinting sulfation in heparin fragments. (C) 2014 The Authors. Published by Elsevier Ltd.
Language
English
Source (journal)
Carbohydrate research. - Amsterdam
Publication
Amsterdam : 2014
ISSN
0008-6215
Volume/pages
400(2014), p. 44-53
ISI
000344880900007
Full text (Publisher's DOI)
Full text (publisher's version - intranet only)
UAntwerpen
Faculty/Department
Research group
Publication type
Subject
Affiliation
Publications with a UAntwerp address
External links
Web of Science
Record
Identification
Creation 13.01.2015
Last edited 04.06.2017
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