Publication
Title
Laboratory investigation of high pressure survival in Shewanella oneidensis MR-1 into the gigapascal pressure range
Author
Abstract
The survival of Shewanella oneidensis MR-1 at up to 1500 MPa was investigated by laboratory studies involving exposure to high pressure followed by evaluation of survivors as the number (N) of colony forming units (CFU) that could be cultured following recovery to ambient conditions. Exposing the wild type (WT) bacteria to 250 MPa resulted in only a minor (0.7 log N units) drop in survival compared with the initial concentration of 10(8) cells/ml. Raising the pressure to above 500 MPa caused a large reduction in the number of viable cells observed following recovery to ambient pressure. Additional pressure increase caused a further decrease in survivability, with approximately 10(2) CFU/ml recorded following exposure to 1000 MPa (1 GPa) and 1.5 GPa. Pressurizing samples from colonies resuscitated from survivors that had been previously exposed to high pressure resulted in substantially greater survivor counts. Experiments were carried out to examine potential interactions between pressure and temperature variables in determining bacterial survival. One generation of survivors previously exposed to 1 G Pa was compared with WT samples to investigate survival between 37 and 8 degrees C. The results did not reveal any coupling between acquired high pressure resistance and temperature effects on growth.
Language
English
Source (journal)
Frontiers in microbiology. - Lausanne, 2010, currens
Publication
Lausanne : Frontiers Research Foundation, 2014
ISSN
1664-302X
Volume/pages
5(2014), 10 p.
Article Reference
612
ISI
000345699800001
Medium
E-only publicatie
Full text (Publisher's DOI)
Full text (open access)
UAntwerpen
Faculty/Department
Research group
Publication type
Subject
Affiliation
Publications with a UAntwerp address
External links
Web of Science
Record
Identification
Creation 13.01.2015
Last edited 05.12.2017
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