Title
Antibody binding to porcine sialoadhesin reduces phagocytic capacity without affecting other macrophage effector functions Antibody binding to porcine sialoadhesin reduces phagocytic capacity without affecting other macrophage effector functions
Author
Faculty/Department
Faculty of Pharmaceutical, Biomedical and Veterinary Sciences. Pharmacy
Publication type
article
Publication
New York ,
Subject
Biology
Human medicine
Source (journal)
Cellular immunology. - New York
Volume/pages
271(2011) :2 , p. 462-473
ISSN
0008-8749
ISI
000296219900032
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Abstract
Sialoadhesin (Sn) is a macrophage-restricted endocytic receptor involved in cell-cell, cell-matrix and cell-pathogen interactions. Recently, porcine Sn (pSn) was shown to be involved in signaling and lately Sn is gaining interest as a potential target for immunotherapy. However, little is known about the effect of ligand binding to Sn on macrophage effector functions. In this study, we tested the effect of antibody binding to pSn on macrophage viability, phagocytosis of microspheres, uptake and processing of soluble antigens, reactive oxygen/nitrogen species production, MHC I and MHC II cell surface expression and cytokine production. This was done by treatment of porcine primary alveolar macrophages with the pSn-specific mAb 41D3, or an isotype-matched control mAb. No significant effect on most effector functions under study was observed, except for a significant reduction of phagocytosis. Thus, antibody binding to pSn can downregulate phagocytosis, which could have implications on homeostasis, infectious and immune diseases, and immunotherapy. (C) 2011 Elsevier Inc. All rights reserved.
E-info
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