Title
Identification of the CD163 protein domains involved in infection of the porcine reproductive and respiratory syndrome virus Identification of the CD163 protein domains involved in infection of the porcine reproductive and respiratory syndrome virus
Author
Faculty/Department
Faculty of Pharmaceutical, Biomedical and Veterinary Sciences. Pharmacy
Publication type
article
Publication
Baltimore, Md ,
Subject
Human medicine
Source (journal)
Journal of virology. - Baltimore, Md
Volume/pages
84(2010) :6 , p. 3101-3105
ISSN
0022-538X
ISI
000275322300044
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Abstract
Scavenger receptor CD163 is a key entry mediator for porcine reproductive and respiratory syndrome virus (PRRSV). To identify the CD163 protein domains involved in PRRSV infection, deletion mutants and chimeric mutants were created. Infection experiments revealed that scavenger receptor cysteine-rich (SRCR) domain 5 (SRCR 5) is essential for PRRSV infection, while the four N-terminal SRCR domains and the cytoplasmic tail are not required. The remaining CD163 protein domains need to be present but can be replaced by corresponding SRCR domains from CD163-L1, resulting in reduced (SRCR 6 and interdomain regions) or unchanged (SRCR 7 to SRCR 9) infection efficiency. In addition, CD163-specific antibodies recognizing SRCR 5 are able to reduce PRRSV infection.
E-info
https://repository.uantwerpen.be/docman/iruaauth/56d79a/37d9177.pdf
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