Title
Functional impairment of PRRSV-specific peripheral <tex>$CD3^{+}CD8^{high}$</tex> cells Functional impairment of PRRSV-specific peripheral <tex>$CD3^{+}CD8^{high}$</tex> cells
Author
Faculty/Department
Faculty of Pharmaceutical, Biomedical and Veterinary Sciences. Pharmacy
Publication type
article
Publication
Paris ,
Subject
Veterinary medicine
Source (journal)
Veterinary research. - Paris
Volume/pages
40(2009) :5 , 15 p.
ISSN
0928-4249
0928-4249
Article Reference
46
Carrier
E-only publicatie
Target language
English (eng)
Full text (Publishers DOI)
Abstract
The replication of porcine reproductive and respiratory syndrome virus (PRRSV) in lungs and lymphoid tissues of PRRSV-infected pigs is already strongly reduced before the appearance of neutralizing antibodies, indicating that other immune mechanisms are involved in eliminating PRRSV at those sites. This study aimed to determine whether PRRSV Lelystad virus (LV)-specific cytotoxic T-lymphocytes (CTL) can efficiently eliminate PRRSV-infected alveolar macrophages. Therefore, CTL assays were performed with PRRSV-infected alveolar macrophages as target cells and autologous peripheral blood mononuclear cells (PBMC) from PRRSV-infected pigs as a source of PRRSV-specific CTL. PBMC of 3 PRRSV-infected pigs were used either directly in CTL assays, or following restimulation in vitro. CTL assays with pseudorabies virus (PRV) Begonia-infected alveolar macrophages and autologous PBMC, from 2 PRV Begonia-inoculated pigs, were performed for validation of the assays. In freshly isolated PBMC, derived from PRRSV-infected pigs, CTL activity towards PRRSV-infected macrophages was not detected until the end of the experiment (56 days post infection - dpi). Restimulating the PBMC with PRRSV in vitro resulted in proliferation of CD3(+)CD8(high) cells starting from 14 dpi. Although CD3(+)CD8(high) cells are generally considered to be CTL, CTL activity was not detected in PRRSV-restimulated PBMC of the 3 pigs until 49 dpi. A weak PRRSV-specific CTL activity was observed only at 56 dpi in PRRSV-restimulated PBMC of one pig. In contrast, a clear CTL activity was observed in PRV Begonia-restimulated PBMC, derived from PRV Begonia-infected pigs, starting from 21 dpi. This study indicates that PBMC of PRRSV-infected pigs contain proliferating CD3(+)CD8(high) cells upon restimulation in vitro, but these PBMC fail to exert CTL activity towards PRRSV-infected alveolar macrophages.
E-info
https://repository.uantwerpen.be/docman/iruaauth/0b9d78/ffc9174.pdf
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