Title
Recombination of two porcine circovirus type 2 strains Recombination of two porcine circovirus type 2 strains
Author
Faculty/Department
Faculty of Pharmaceutical, Biomedical and Veterinary Sciences. Pharmacy
Publication type
article
Publication
Wien ,
Subject
Human medicine
Source (journal)
Archives of virology. - Wien
Volume/pages
154(2009) :5 , p. 875-879
ISSN
0304-8608
ISI
000265915300017
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Abstract
Pigs can be concurrently infected with different PCV2 strains. In this study, a cell-culture-adapted PCV2 strain, originating from a PMWS-affected pig, was purified by limiting dilution. Three different strains were obtained, and one of them was a perfect mosaic of the other two, with recombination breakpoints in ORF1 and ORF2. Incongruence was observed between phylogenetic trees constructed with the whole genome, ORF1 and ORF2. Amplification of ORF1 and ORF2 from original material, followed by cloning and sequencing, resulted in sequences corresponding to the parental strains, but not with the mosaic strain. These results demonstrate that PCV2 can undergo recombination.
E-info
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